COURSE OUTLINE & SYLLABUS

Students in this course will become proficient in techniques of fluorescence microscopy for investigation of biological and non-biological samples, but particularly for the identification of microbes in the environment. The course consists of demonstration plus hands-on training in the practice of sample collection, preparation, and observation using the technique of fluorescence microscopy. After completion of this course students will have gained experience in designing protocols using fluorescence microscopy, and in implementing those protocols for the investigation of lab and/or field samples.

Day 1:
•Morning
Course introduction; introduction to the theory and practice of epifluorescence microscopy, microscopes for fluorescence, filter sets (identifying, purchasing, installing), light sources (replacing and aligning burners), cleaning, alternate light source for fluorescence.

•Afternoon
The practice of fluorescence microscopy: Labs 1, 2: Sample preparation. Intrinsic fluorescence, Survey of fibers

Day 2:
•Morning
Beginning fluorescence investigation: Labs 3, 4: Survey of organisms, Cytochemical fluorescence microscopy

•Afternoon
Investigation of biological samples. Labs 57: Plant and fungi cell walls, Lipids, Insta-Fluor slides for bacteria

Day 3:
•Morning
Labs 8, 9: ID bacteria using SYTO, Testing liquids for bacteria

•Afternoon
Lab 10, 11: Bacteria viability, Fluorescent staining of polysaccharides, Unknowns.

There is no prerequisite for this course.

Note: This course runs 8:00 am to 5:00 pm on all days.