COURSE OUTLINE & SYLLABUS

Students will acquire the basic knowledge and practical skills to apply fluorescence methods in their areas of interest. The majority of the course will be hands-on demonstration and training in the practice of sample preparation for epifluorescence microscopy.

There is no prerequisite for this course.

Day 1: Course Introduction

• Introduction to the theory and practice of epifluorescence microscopy
• Introductory fluorescence investigations
• Microscopes for fluorescence
• Filter sets and cleaning

Day 2: The practice of fluorescence microscopy:

• Pitfalls and shortcomings of fluorescence microscopy
• Intrinsic, general and specific fluorescence probes
• Viability probes
• Sample preparation
• Dye loading

Day 3: Continued practice:

• Problem solving
• Resources, materials and probes

Note: This course runs 8:00 am to 5:00 pm on all three days